صفحه محصول - پاورپوینت Photosynthesi The Hill Reaction

پاورپوینت Photosynthesi The Hill Reaction (pptx) 24 اسلاید

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تعداد اسلاید: 24 اسلاید

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Photosynthesis II: The Hill Reaction What’s the point of this lab? Understand the electron transport chain Alter variables in the ETC to observe outcome Develop methods to determine order of events in the ETC Reminders 6CO2 + 12H2O + light  C6H12O6 + 6O2 + 6H2O Excitation Chlorophyll (water split) ETC (PQ as H+ pump)  Excitation Chlorophyll  ETC  Reduction NADP+ to NADPH + H+ (stroma) High H+ concentration in thylakoid interior causes ATP production (stroma) Electron Transport Chain DCPIP – the electron theif High absorbance at 600 nm Blue = Oxidized (electron poor) Clear = Reduced (electron rich) Hill Reaction (Figure 7.1) Review of Hill Reaction Isolated chloroplasts could be stimulated by light to transfer e- through ETC to an artificial electron acceptor We measure the Hill Reaction with other e- acceptors How do herbicides affect the ETC? DCPIP: artificial e- acceptor from ETC Herbicide: blocks ETC Hill Reaction http://www.life.uiuc.edu/govindjee/photoweb/NewZScheme.jpg DCPIP (Hill Reaction) DCPIP Blue Oxidized (no e-) Colorless Reduced (gains e-) If Photosystem II is not stopped, and DCPIP is allowed to steal electrons and unclog the e- back-up in the ETC, then H2O is split into 2 H+ and O2 and e-'s are moved down the chain. Electron Transport Chain